Table I.

Effects of ICE and CPP32 peptide inhibitors on Ad22 induced annexin V bindinga

Percentage of Annexin V Positive Cellsb
1 hour3 hours6 hours
MOPC-21Ad22MOPC-21Ad22TEPC 183CH11
− Inhibitor8.7 ± 2.949.1 ± 1.99.2 ± 4.252.9 ± 5.87.9 ± 1.828.8 ± 0.2
+ DMSO (0.5%)c9.9 ± 1.748.5 ± 4.811.9 ± 4.462.5 ± 3.57.8 ± 0.733.6 ± 2.7
+ Ac-YVAD-CMK10.2 ± 1.748.1 ± 1.213.5 ± 1.054.6 ± 1.88.8 ± 1.711.5 ± 2.6
+ Z-VAD-FMK9.2 ± 3.445.7 ± 9.57.8 ± 0.0158.4 ± 4.910.4 ± 0.49.8 ± 3.3
+ Ac-DEVD-CHO8.3 ± 1.647.8 ± 8.68.3 ± 1.862.0 ± 4.08.4 ± 2.817.8 ± 2.7
  • a Jurkat E6 cells were preincubated with the indicated ICE/CPP32 inhibitors (100 μM) for 60 min before further culture with 1 μg/ml mAb Ad22, CH11, or isotype control as indicated. Cultures with CH11 were included as controls.

  • b Annexin V-FLUOS binding was assessed by flow cytometry. Results are expressed as the mean of to separate experiments ± SD.

  • c Mock control.