Table I.

Cytokine production by T cell clonesa

IL-2IFN-γIL-4IL-10
CD1NTCD1NTCD1NTCD1NT
14S.3>80,00051014,235
14S.4>80,0002,83811,877
14S.7>80,0007022,26721,347
14S.107321>80,000951,93121,846
24.716,927>80,0008,482
24.83,88113,5394,860166
  • a T cells were cocultured with RMA-S.CD1d1 or RMA-S cells. The supernatants were sampled at 24 and 48 h poststimulation and stored at −20°C until cytokine quantitation was done by ELISA (IFN-γ, IL-2, IL-4, and IL-10). Results are shown as picograms per 106 T cells/milliliter. The patterns of cytokine secretion were similar for both time points. AE7, an I-Ak-restricted T cell clone specific for pigeon cytochrome c, was stimulated with irradiated splenocytes, in either the presence or the absence of pigeon cytochrome c. AE7 T cells and APCs did not secrete any detectable cytokines in the absence of Ag. In the presence of Ag, IL-2 (5, 323 pg/ml) and IFN-γ (>40,000 pg/ml) were produced. No IL-4 or IL-10 was detected. —, not detected (level of sensitivity was <78 pg/ml). Similar results were obtained in two to four experiments.