Table I.

P38 inhibitor blocks CpG DNA-mediated cytokine productiona

MediumAnti-IgMCpG DNANonCpG DNAPMA + Ionomycin
TNF-αIL-6TNF-αIL-6TNF-αIL-6TNF-αIL-6TNF-αIL-6
MediumND902811864783596ND11412564773
SB202190 (5 μM)ND4025223641704ND228221464
SB202190 (10 μM)NDND77177ND382NDND6101890
  • a WEHI-231 cells (106 cells/ml for IL-6 and 107 cells/ml for TNF-α) were pretreated with SB202190 (5 or 10 μM) for 1 h and then stimulated with medium, CpG or non-CpG DNA (1 μM), anti-IgM (10 μg/ml), or PMA (100 ng/ml) plus ionomycin (1 μM) for 4 h (for TNF-α) or 12 h (for IL-6). Culture supernatants were analyzed by ELISA for TNF-α or IL-6 (pg/ml). ND stands for not detected. Data (pg/ml) represent the mean of triplicates. SE was <10%. The experiment was repeated three times with similar results.