Table II.

In vitro T cell reactivity to islet antigens in transgenic and non-transgenic IL-10-NOD micea

Expt.Age (wk)IL-10 tgDiabetesIn Vitro Lymphocyte Proliferative Response (SI ± SD) to
Bcgr.cpmbGAD65hsp65Insulin
15++34322.64 ± 0.306.89 ± 1.560.91 ± 0.11
25+42425.09 ± 1.123.60 ± 0.491.40 ± 0.47
26++66642.83 ± 0.603.51 ± 0.451.28 ± 0.87
26+82054.53 ± 0.794.23 ± 0.561.43 ± 0.31
36+65608.48 ± 0.882.66 ± 0.511.92 ± 0.28
36++67745.66 ± 0.553.22 ± 0.191.36 ± 0.39
48++56431.28 ± 0.334.99 ± 0.86ND
Mean SI4.36 ± 2.384.16 ± 1.421.38 ± 0.32
1563863.54 ± 0.352.79 ± 0.510.79 ± 0.23
1566663.98 ± 0.232.00 ± 0.200.72 ± 0.29
2617571.28 ± 0.161.64 ± 0.481.25 ± 0.02
2641102.06 ± 0.694.76 ± 1.131.61 ± 1.01
3641073.50 ± 0.333.14 ± 1.390.86 ± 0.10
3615703.05 ± 0.292.63 ± 0.531.02 ± 0.23
4865491.89 ± 0.172.88 ± 0.28ND
Mean SI2.76 ± 1.012.83 ± 1.000.89 ± 0.34
(p = 0.42)(p = 0.06)(p = 0.11)
  • a In vitro proliferative responses of splenocytes from individual mice against GAD65 (40 μg/ml), hsp65 (20 μg/ml), and porcine insulin (40 μg/ml). The IL-10-NOD mice (N2 and N3 backcross generations) were generated by backcrossing of IL-10-BALB/c mice with NOD-shi mice for 2 to 3 generations. p values were calcuated by unpaired students’ t test. The p values <0.05 are considered significant. SI, stimulation index. ND, not determined.

  • b Bcgr. cpm represent the cpm obtained with unstimualted lymphocyte cultures.