Table I.

Transendothelial migration of peripheral blood T cells in response to RANTES.her2.IgG3a

ConditionConcentration (ng/ml)% Migration (migration index)bAverage Migration Index ± SEM
Expt. 1Expt. 2Expt. 3Expt. 4
None12.9 (1.0)16.4 (1.0)5.2 (1.0)5.9 (1.0)1.0 ± 0.00
rRANTES0.124.9 (1.9)23.0 (1.4)9.2 (1.8)8.4 (1.4)1.6 ± 0.13
1.034.4 (2.7)27.5 (1.7)14.3 (2.8)9.9 (1.7)2.2 ± 0.30
10.0NDND7.6 (1.5)6.8 (1.2)1.3 ± 0.11
RANTES.her2.IgG30.128.2 (2.2)26.7 (1.6)6.7 (1.3)8.1 (1.4)1.6 ± 0.20 (p = 0.2665)c
1.038.3 (3.0)34.4 (2.1)15.5 (3.0)13.3 (2.2)2.6 ± 0.24 (p = 0.0133)c
10.044.9 (3.5)6.2 (1.6)15.1 (2.9)14.3 (2.4)2.6 ± 0.40 (p = 0.0062)c
her2.IgG30.118.5 (1.4)6.7 (0.4)6.6 (1.3)9.6 (1.6)1.2 ± 0.27
1.018.1 (1.4)10.0 (0.6)9.6 (1.9)9.6 (1.6)1.4 ± 0.27
10.018.1 (1.4)6.7 (0.4)8.9 (1.7)5.9 (1.0)1.1 ± 0.28
  • a A HUVEC monolayer was grown to confluence on the porous membrane of a transwell plate (see Materials and Methods). Peripheral blood T cells were purified from blood obtained from normal donors and plated in the upper well of the transwell plate. rRANTES, RANTES.her2.IgG3, or her2.IgG3 were added to the lower wells at the indicated concentrations. Migration was allowed to proceed at 37°C for 24 h, and the number of migrated cells in the lower well was counted and recorded as percent migration.

  • b Values correspond to percent of T cells placed in the upper well (1 × 105 in expt. No. 1, 4.7 × 104 in expt. No. 2, 2 × 105 in expts. No. 3 and No. 4) that migrate to the lower well in response to the described conditions. In parentheses, we show migration index for each experiment calculated as percent migration for a specific condition divided by control percent migration in medium only.

  • c The p value was calculated from paired t test comparing RANTES.her2.IgG3 to her2.IgG3 in all four experiments at the same concentration.