Table II.

Down-regulation of CD1 is independent of secreted factorsa

Upper ChamberLower ChamberCD1+ Cells (lower chamber)
Infected CD1+ cellsUninfected CD1+ cells55
Uninfected CD1+ cellsInfected CD1+ cells17
Uninfected CD1+ cellsUninfected CD1+ cells57
  • a Infected cells (1 × 106) were plated in the upper chamber of a transwell system. In the lower chamber, 3 × 106 uninfected CD1+ APC were cultured. After 48 h, the cells in the lower chamber were detached with EDTA, and FACS staining for CD1b was performed. One representative experiment of six is shown.