Table I.

Down-regulation of CD1 is independent of cytokinesa

M. tuberculosisAbs AddedCytokines AddedCD1+ Cells (%)
None58
+IL-10None16
+TGF-βNone13
+IL-6None15
+IL-12None10
+TNF-αNone13
+NoneNone12
NoneIL-1056
NoneTGF-β55
NoneIL-659
NoneIL-1254
NoneTNF-α54
  • a CD1+ cells were infected with live M. tuberculosis at an MOI of 10. After 4 h, extracellular bacteria were washed off and the incubation of the cells was continued in the presence of Abs against a panel of cytokines (all at 20 μg/ml). After 48 h, cells were harvested and CD1 expression on the cell surface was analyzed by FACS. Uninfected CD1+ cells were, incubated in the presence of a panel of cytokines (all at 10 ng/ml). After 48 h cells were harvested and CD1 expression on the cell surface was analyzed by FACS. Data represent one experiment out of four with similar results.