Table III.

Effects of anti-IL-12 and anti-IFN-γ mAb treatment on T cells in adult IL-10−/− mice

mAb TreatmentaNumber of CD4+ T CellsFrequency of Colonic CD4+ T CellsbNumber of Cells Spontaneously Producing IFN-γ per 5 × 105 MLN CellscIFN-γ Production per 2 × 105 CD4+ MLN T Cells (ng/ml)dMHC II Expression on Colonic Epithelial Cellse
Spleen (×10−6)MLN (×10−6)
Anti-IL-128.6 ± 3.95.1 ± 4.8g35 ± 7g0.4 ± 1.4g1.1 ± 0.4
Anti-IFN-γ5.6 ± 2.95.6 ± 2.8g61 ± 15NDND
Control mAb7.5 ± 3.110.5 ± 5.961 ± 1642 ± 221.0 ± 0.3+
WTf3.9 ± 0.60.27 ± 0.0322 ± 6NDND
  • a Mice treated with 2 mg mAb per week for 8 wk.

  • b Mean number of colonic CD4+ T cells per 10 to 15 high power-fields (×400) assessed by immunohistochemistry.

  • c Spontaneous IFN-γ production was assayed by ELISPOT.

  • d IFN-γ production was assayed by ELISA following in vitro stimulation and correlated to the absolute number of CD4+ T cells in each culture.

  • e MHC class II expression by colonic epithelial cells was assessed by immunohistochemistry.

  • f Values for unmanipulated 129/SvEv WT mice (n = 6).

  • g Denotes a significant difference to corresponding control mAb score (assessed by using an unpaired Student’s t test).