Table II.

Inhibition of percentage of specific cytotoxic activity of CD4+ T cells by mAb to TRAIL and Fasabc

Target CellsT Cell Clones
A4C2 (%)C5C4 (%)C5C5 (%)2C4 (%)CO (%)
Autol Mel-FH53236584
Autol Mel-FH+ M 180 (anti-TRAIL)40 (25)8 (65)60 (8)82 (2)
Autol Mel-FH+ M3 (anti-Fas)51 (4)21 (9)65 (0)80 (5)
Autol Mel-FH+ Isotype control53 (0)23 (0)64 (0)82 (1)
Allo Mel-RM8176776685
Allo Mel-RM+ M18070 (14)72 (5)65 (16)63 (5)87 (0)
Allo Mel-RM+ M382 (0)76 (0)72 (0)66 (0)89 (0)
Allo Mel-RM+ Isotype control82 (0)75 (0)75 (0)65 (0)86 (0)
Mel-CV6916341484
Mel-CV + M18058 (16)5 (69)19 (44)10 (40)71 (15)
Mel-CV + M368 (1)19 (0)28 (18)17 (0)81 (4)
Mel-CV + Isotype control68 (1)18 (0)33 (1)16 (0)84 (0)
  • a M180 and M3 mAbs and isotype control used at 20 μg/ml initially and left in culture for the 16-h assay diluted to 5 μg/ml. Figures in parentheses indicate percentage of inhibition. Autol, autologous; Allo, allogeneic.

  • b In the same assay, specific cytotoxicity induced by FasL and TRAIL on Jurkat T cells was 73 and 85%, respectively. MAb M180 inhibited TRAIL- and FasL-induced specific cytotoxicity by 73 and 9%, respectively. MAb M3 inhibited Fas- and TRAIL-induced specific cytotoxicity by 99 and 0%, respectively.

  • c SEs for all values were <3%. Percentage of inhibition, in the presence of mAb, >5% was significantly different by t test of the data.