Table III.

IFN-γ and IL-10 levels measured on days 4, 8, and 15 post induction in spleen cell culture supernatants derived from GVHD mice with grafts from either wild-type or IFN-γ gko donors, and from recipient mice that did not receive grafts (controls)

Recipient GroupDay PostinductionNumber of Mice/GroupUnits of Cytokine (U/ml) ± SEMa
IFN-γIL-10
Wild-type graft43< .514.5 ± 1.7
8321.6 ± 0.9< 0.2
153< 0.5< 0.2
IFN-γ gko graft43< 0.57.0 ± 1.0
83< 0.5< 0.2
153< 0.5< 0.2
No graft (control)3< 0.5< 0.2
  • a The values < 0.5 and < 0.2 indicate levels below which IFN-γ and IL-10, respectively, could not be measured reliably by ELISA.