Table III.

Induction of IL-6 and LIF production by endogenous IL-17 produced by RA synovium piecesa

ActivatorsIL-6 (ng/ml)LIF (ng/ml)
Medium00.05 ± 0.01
IL-1715.49 ± 2.410.98 ± 0.07
IL-17 + mAb500.06 ± 0.01
IL-17+ MX114.63 ± 3.8ND
IL-1154.60 ± 13.481.64 ± 0.20
IL-1 + mAb5145.95 ± 22.51.62 ± 0.18
RA 54 supernatant151.3 ± 11.091.20 ± 0.04
RA 54 supernatant + mAb552.7 ± 9.100.54 ± 0.01
  • a Cytokines (IL-1β, 100 pg/ml, or IL-17, 50 ng/ml) or synovium piece supernatants were preincubated in the presence or absence of 1 μg/ml of a control mAb (MX1) or 1 μg/ml of anti-IL-17 (mAb5). The mixture was incubated with synoviocytes for 12 h. Plates were washed before addition of fresh medium. IL-6 and LIF levels were determined by ELISA in the 48-h supernatants.