Table I.

Regulation by IL-4, IL-13, and IL-10 of IL-6 production by RA synoviocytes induced by IL-1β and IL-17a

ActivatorsnIL-6 Production (ng/ml)
0+ IL-4+ IL-13+ IL-10
069.8 ± 2.844.7 ± 6.3*+356b43.2 ± 12.4*+340b11.2 ± 1.6**+14b
IL-14496.9 ± 55.1667.2 ± 67.9***+34682.8 ± 93.1***+37322.4 ± 61.8***−35
IL-174282.7 ± 44.2357.8 ± 61.7***+27382.9 ± 55.6***+35179.2 ± 62.6***−37
IL-1+ IL-174597.3 ± 57.8726.3 ± 39.9***+22798.7 ± 80.1***+34530.4 ± 47.1***−11
  • a 104 Synoviocytes were incubated in 96-well culture plates for 7 days with IL-1β (100 pg/ml) or IL-17 (20 ng/ml) or their combination in the presence of 50 ng/ml of IL-4, IL-10, or IL-13. After 7 days of culture, IL-6 levels were measured by ELISA and are expressed as the mean ± SEM of nseparate experiments. Differences in IL-6 production between the IL-4-, IL-13-, or IL-10-treated group and the control group were analyzed with the nonparametric Wilcoxon paired t test. *p < 0.005; **p < 0.01; ***p < 0.0001.

  • b Column represents % induction.