Table III.

In vitro ISS-ODN stimulation of anti-CD3-induced T cell cytokine production: effect of neutralizing Abs to innate cytokines (IL-12 and IFNs)a

StimulationIL-3 (pg/ml)IL-5 (pg/ml)GM-CSF (pg/ml)
Anti-CD3/ISS-ODN1183 ± 217730 ± 230535 ± 118
Anti-CD3/ISS-ODN and anti-IL-121154 ± 209870 ± 263829 ± 153b
Anti-CD3/ISS-ODN and anti-IFN-γ1169 ± 2142218 ± 231b840 ± 222b
Anti-CD3/ISS-ODN and anti-IFN-α/β1565 ± 228b753 ± 215682 ± 122b
  • a Splenocytes (5 × 106/ml) were incubated with ISS-ODN or M-ODN (10 μg/ml) at 37°C for 2 h and then added to anti-CD3-coated plates (5 × 105 cells/well) in triplicate, with or without neutralizing Abs to mouse IL-12, IFN-γ, and IFN-α (Biosource). The cytokine profiles of the supernatants were assayed by ELISA. Results are expressed as the mean ± SE (n = 4 per group).

  • b Denotes p < 0.05, anti-CD3 plus ISS-ODN treated group vs anti-CD3 plus ISS-ODN plus cytokine neutralizing Ab-treated group.