Table II.

Effect of in vitro ISS-ODN stimulation on anti-CD3-induced T cell cytokine productiona

StimulationIL-3 (pg/ml)IL-5 (pg/ml)GM-CSF (pg/ml)
Anti-CD32495 ± 1211553 ± 1201090 ± 141
Anti-CD3/ISS-ODN1183 ± 217b730 ± 230b535 ± 118b
Anti-CD3/M-ODN4975 ± 8481795 ± 1161951 ± 538
  • a Splenocytes (5 × 106/ml) were incubated with ISS-ODN or M-ODN (10 μg/ml) at 37°C for 2 h and then added to anti-CD3-coated plates (5 × 105 cells/well) in triplicate. The cytokine profiles of the supernatants were assayed by ELISA. Results are expressed as the mean ± SE (n = 4 per group).

  • b Denotes p < 0.05, anti-CD3 plus ISS-ODN-treated group vs. anti-CD3-treated group.