Table I.

In vivo ISS-ODN administration of OVA-sensitized mice inhibits IL-5 without induction of IFN-γa

GroupTreatmentIL-3 (pg/ml)IL-5 (pg/ml)IFN-γ (pg/ml)
AControl1299 ± 89657 ± 52< 20
BISS-ODN (-1d)309 ± 26b112 ± 18b< 20
CISS-ODN (-3d)463 ± 48b144 ± 27b< 20
DM-ODN (-1d)964 ± 81508 ± 77< 20
  • a Female BALB/c mice were sensitized to develop a Th2 response to OVA as detailed in Fig. 2, but without subsequent aerosol OVA challenge. Eight weeks after the last OVA/alum injection, mice were injected i.p. with 100 μg of ODN, 1 day (-1d) or 3 days (-3d) prior to their sacrifice. The cytokine profiles of the supernatants derived from splenocytes incubated for 72 h with 100 μg/ul of OVA were assayed by ELISA. Only baseline levels of IL-3, IL-5, and IFN-γ were detected in the supernatants of cultures incubated without OVA. No measureable levels of IL-4 and GM-CSF were detected in the various supernatants. Results are expressed as the mean ± SE (n = 4 for each group).

  • b Denotes p < 0.05, ISS-ODN-treated group vs control.