Table I.

Recognition of a tyrosinase cDNA fragment 12F4 by HLA-A1-restricted TIL1388a

StimulatorHLA-A1TyrosinaseTIL1388, IFN-γ (pg/ml)
None<10
Skme123++1566
293A1+<10
586mel+<10
624mel+<10
624melA1++269
COS+ HLA-A1+<10
COS+ HLA-A1+ 12F4++1729
  • a Recognition by TIL was tested by measuring IFN-γ secretion using ELISA. 293A1 is a embryonic kidney cell line stably transfected with HLA-A1 cDNA. 624melA1 is a melanoma cell line stably transfected with HLA-A1 cDNA. COS cells were transfected with HLA-A1 cDNA alone or with both HLA-A1, cDNA, and the 12F4 cDNA clone, a tyrosinase cDNA fragment isolated by cDNA expression cloning.