Table III.

Vaccine-induced CD8⁺ T cell responses to melanoma-associated Ags

Patient ID	gp100	Tyrosinase	Melan-A/MART-1	MAGE-3	TRP-2	MC1R	Any Peptide
191	6.1^a	0^b	0	0	0	0	+
375	0	0	45.5	89.2	0	0	+
418	0	0	8.3	10.6	0	0	+
422	0	0	0	11.8	0	0	+
436	10.7, −10.3^c	22.4, −6.3	0	0	36.1, 16.6^d	23.4, 13.7	+
442	0	0	0	8.2, 5.8	0	0	+
443	−5.5	0	0	0	0	0	−
445	0	0	0	0	0	0	−
458	7.7	0	11.7	0	0	22.2	+
460	13.0	11.5	0	0	0	0	+
462	0	0	0	0	0	0	−
465	0	0	0	0	0	0	−
467	0	21.8, 6.8	0	0	0	0	+
468	69.5, 8.4	0	0	0	37.1, −19.0	0	+
471	0	0	0	0	0	0	−
475	0	0	0	−17.6	0	0	−
477	0	0	0	0	0	0	−
478	0	17.1	12.7	0	−6.4	0	+
479	0	13.4	0	0	15.4	0	+
482	14.8	0	0	0	0	0	+
483	0	0	0	0	0	0	−
484	0	0	0	0	0	0	−

%	27%	23%	18%	18%	13%	9%	59%

a Number of vaccine-induced peptide-specific CD8⁺ T cells/500,000 PBL. These were calculated by subtracting the number of peptide-specific CD8⁺ T cells present prior to immunization from that after four immunizations in the same patient.
b 0, Values that were between −4.9 and 4.9 after subtraction of the preimmunization response. This range on either side of 0 is approximately twice the maximum standard deviation of our assay.
c A value with a − indicates that the pre-immunization response was greater than the postimmunization response.
d Multiple values indicate stimulation of CD8⁺ T cells to more than one peptide on the same Ag.