Table I.

Effects of IFN-γ treatment on early cytokine production by Mφ from normal and lupus-prone murine strainsa

Mφ StrainIFN-γCytokine Production
IL-1 (U/ml × 10−3)IL-6 (ng/ml)IL-10 (U/ml × 10−3)
BALB/c127 ± 15827 ± 990 ± 10
+56 ± 2b818 ± 191 ± 0b
A/J199 ± 35712 ± 11450 ± 1
+19 ± 0.7b526 ± 11b10 ± 2b
C57B1/622 ± 2318 ± 7190 ± 10
+5 ± 0.2b350 ± 1421 ± 3b
MRL/++30 ± 3286 ± 3630 ± 1
+21 ± 3174 ± 16b0b
NZB/WF124 ± 5576 ± 2435 ± 16
+1 ± 0.3b570 ± 2190 ± 8b
  • a Peritoneal Mφ (105) from each murine strain were cultured with LPS (100 ng/ml) in the absence or presence of IFN-γ for 16 h, and cell lysates were analyzed for IL-1 bioactivity using the D10.G4 bioassay and conditioned medium analyzed for IL-6 and IL-10 by ELISA.

  • b Significantly (p < 0.01) different from values of cytokine produced in the absence of IFN-γ.