Table I.

Lymphocytes responses to different concentrations of cross-linked anti-CD3 mAb or Con Aa

Stimulus[3H]Thymidine Incorporation (cpm)
UninfectedbT. gondii infectedc
CD3 mAb/ml
5.0 μg122,520 ± 1,96924,592 ± 654
2.0 μg96,250 ± 5,44911,877 ± 1,583
0.5 μg77,365 ± 2,7814,930 ± 245
ConA/ml
5.0 μg106,850 ± 3,03913,575 ± 77
2.0 μg74,299 ± 1339,521 ± 77
0.5 μg35,575 ± 1,3765,286 ± 457
  • a Proliferative response to cross-linked anti-CD3 mAb or Con A of lymphocytes from CBA/J mice (n = 3/group) infected with T. gondii (PLK) tachyzoites (1.2 × 105/mouse) or age-matched uninfected mice. On day 5 after infection, lymphocytes from spleens of infected mice or control mice were cultured in quadruplicate (2 × 105 viable cells per well) in flat-bottom wells in RPMI 1640 plus 10% FCS. The cells were cultured in the presence of different concentrations of either anti-CD3 mAb (5, 2, and 0.5 μg/ml) or Con A (5, 2, and 0.5 μg/ml). Cells from uninfected and infected animals were cultured in medium only, and cpm in uninfected group is 2142 ± 145 and experimental group is 2250 ± 280. [3H]Thymidine incorporation was determined on day 3 of culture. The results are expressed as means ± SD for quadruplicate. These results are representative of three separate experiments.

  • b,c Significant difference in the levels of lymphoproliferation between uninfected (b) and infected mice (c) (b vs c, p < 0.005).