Table I.

Hsp27 expression of PBMC before and after heat shock

Cell PhenotypeMean Fluorescence Intensitya
Before heat shockAfter heat shock
CD14+ monocytes25117
CD20+ B cells4140
CD4+ T cells483
CD8+ T and NK cells458
CD16+ T and NK cells353
  • a Protein expression was analyzed by flow cytometry of stained permeabilized cells before or 14 h after heat shock for 1 h at 42°C. Background staining with an IgG control Ab gave MFI values in the range of 2 to 4 for gated monocytes and lymphocytes before or after heat shock.