Table I.

Costimulation of IL-2 production and proliferation by B7.1 and B7.2 is additive

Concentration Anti-CD3 mAb (μg/ml)Mean (± SEM)aMeasured Response
B7.1 Ig (5 μg/ml)B7.2 Ig (25 μg/ml)B7.1 Ig + B7.2 Ig
0.455.5 (± 4.9)66.7 (± 6.7)125.4 (± 10.5)[3H]Thymidineb incorporation
0.42.0 (± 0.4)0.84 (± 0.3)3.0 (± 0.3)IL-2c production
109.4 (± 0.4)5.2 (± 1.2)13.3 (± 3.3)
  • a Averages ± SE of three independent experiments.

  • b [3H]Thymidine incorporation expressed as cpm × 10−3.

  • c IL-2 values expressed as U/25,000 cells/ml.