Table I.

Sequence of the oligonucleotides used for RT-PCR and hybridization

TargetPrimer SiteSequence
β-actinSense5′-ATGGATGACGATATCGCTGC-3′
Antisense5′-GCTGGAAGGTGGACAGTGAG-3′
Probe5′-CGTACCACAGGCATTGTGATGG-3′
mMCP-1, -2, -4Sense5′-GCTGGAGCTGAGGAGATT-3′
Antisense5′-GGTGAAGACTGCAGGGG-3′
mMCP-1Probe5′-AGTTAAAAACAGCATACATGG-3′
mMCP-2Probe5′-TAAGAACGGTTCGAAGGAGAGG-3′
mMCP-4Probe5′-TGAGAGAGGGTTCACAGCTAC-3′
mMCP-5Sense5′-GAACTACCTGTCGGCCTGCAG-3′
Antisense5′-AGAACCTTCTGGAAGCTCAGG-3′
Probe5′-GTCCACGACATCATGCTACTG-3′
mMCP-7Sense5′-CCTCACTGTGTCCAAATGCTA-3′
Antisense5′-CCTCCTGCCTCAGAGACC-3′
Probe5′-GGCAGGTGAGCCTGCGTGCCA-3′
Granzyme BSense5′-CTCCACGTGCTTTCACCAAA-3′
Antisense5′-GGAAAATAGTACAGAGAGGCA-3′
Probe5′-GCTCTGCTTCCCCTCAGTGCC-3′
FcεRIαSense5′-TTAGCACCTGAAGGTGCAGGG-3′
Antisense5′-ACATGAGTGGCCTTTGACAGT-3′
Probe5′-GGTACCGTCTCTGAGGTGAAC-3′
FcεRIβSense5′-AAATGGACACAGAAAATAGGAGC-3′
Antisense5′-GGCATCTGCAGAACAATCCAA-3′
Probe5′-GTCTGCTCCGTACTCTATTGG-3′
SCFSense5′-TGGAGCTCTAGAACAGCTAAACGG-3′
Antisense5′-GGCTGTCTAGAGTAGGCTGGAGTC-3′
Probe5′-GCATCTGACACTAGTGACTGT-3′
IL-9Sense5′-GTGCTGGGCCAGAGATGCAGC
Antisense5′-GCATCTGTCTTCATGGTCGGC-3′
Probe5′-GCCTGTTTTCCATCGGGTGAAA-3′