Table I.

Effect of IL-10 on IgE and IgG4 production by purified tonsillar B cellsa

StimuliIL-10Unseparated B CellsIgD+ B Cells
IgE (ng/ml)IgG4 (ng/ml)Proliferation (cpm × 10−3)IgE (ng/ml)IgG4 (ng/ml)
None<<1.5 ± 0.2<<
+<58 ± 61.4 ± 0.3<<
IL-4<<1.6 ± 0.2<<
+<110 ± 101.5 ± 0.3<<
Anti-CD40<<4.2 ± 0.6<<
+<418 ± 959.5 ± 0.8<<
IL-4+ Anti-CD4098 ± 18331 ± 598.1 ± 0.920 ± 550 ± 11
+648 ± 1501579 ± 15036.5 ± 6120 ± 20600 ± 120
  • a Unseparated and naive IgD+ tonsillar B cells were either unstimulated or stimulated with IL-4, anti-CD40 mAb, or IL-4 plus anti-CD40 mAb in the absence or presence of 50 ng/ml IL-10. IgE and IgG4 were quantified in day 12 supernatants. The proliferation of unseparated B cells was measured at day 3. Results are expressed in cpm × 10−3. Data are means ± SD of quintuplicate values and are representative of one of four experiments.