Table II.

Absence of lysis of non-HLA-A2 OLs mediated by HLA-A2 MBP peptide-reactive CD8 T cell linesa

CD8 T Cell Line HLA-HaplotypeOLOL + Pan-MHC Class I AbOL + IgG2aHMy2 Cells + MBP Peptide
Control group CD8 T cell lines
A2,-:B18,44**c3%5%4%57%
A2,-:B18,44**c6%5%7%52%
A2,32:B44,b2%2%2%44%
A2,24:B7,44***c5%5%6%45%
A2,24:B7,44***c4%5%5%42%
MS patients CD8 T cell lines
A2,1:B8,275%1%2%46%
A2,1:B8,573%3%3%42%
A2,-:B18,515%5%7%52%
  • a Data indicate the cytotoxic effect (% 51Cr release) induced by MBP peptide 110-118-reactive CD8 T cell lines, generated from HLA-A2 normal donors and MS patients, on human OLs derived from a non-HLA-A2 donor (HLA-A28,33:B44,14). OL targets were utilized alone (OL) or in the presence of anti-MHC class I mAb or IgG2a control Ab. Data present results from individual cell line experiments conducted at 10:1 E:T ratios. The corresponding extent of lysis of HLA-A2-transfected Hmy.C1R cells that were pulsed with MBP peptide 110-118 mediated by individual CD8 T cells is also shown.

  • b , **, and *** indicate CD8 T cell lines that were used in the studies presented in Table I.

  • c and c indicate cell lines derived from the same donor.