Table I.

MBP peptide-reactive CD8 T cell line-mediated cytotoxicity of HLA-A2 OL in absence of exogenous peptidea

CD8 T Cell Line HLA-HaplotypeOLOL + Pan-MHC Class I AbOL + IgG2aHMy2 Cells + MBP Peptide
Control group CD8 T cell lines
A2,32:B44,b21%5%17%65%
A2,25:B7,1815%3%14%47%
A2,-:B18,44**18%5%15%43%
A2,31:B60,9%2%10%43%
A2,24:B7,44***14%2%14%43%
MS patients CD8 T cell lines
A2,1:B8,628%2%6%38%
A2,-:B7,20%5%18%49%
  • a Data indicate the cytotoxic effect (% 51Cr release) induced by MBP peptide 110-118-reactive CD8 T cell lines, generated from HLA-A2 normal donors and MS patients, on human OLs derived from an HLA-A2 donor (HLA-A2,28:B35,45). OL targets were utilized alone (OL) or in presence of anti-MHC class I mAb or IgG2a control mAb. Data present results from individual cell line experiments conducted at 10:1 E:T ratios. The corresponding extent of lysis of HLA-A2-transfected HMy2.C1R cells that were pulsed with MBP peptide 110-118 mediated by individual CD8 T cell lines is also shown.

  • b , **, and *** indicate which T cell lines are the same as those used in the studies presented in Table II.