Table I.

Binding capacity of single Arg insulin B(5–15) analogues for DQA1*0102/DQB1*0602a

PeptideSequenceIC50 (μM)
Insulin B(5–15)HLCGSHLVEAL1.6
Insulin B(5–15)5RRLCGSHLVEAL1.5
Insulin B(5–15)6RHRCGSHLVEAL>100
Insulin B(5–15)7RHLRGSHLVEAL2.5
Insulin B(5–15)8RHLCRSHLVEAL25
Insulin B(5–15)9RHLCGRHLVEAL>100
Insulin B(5–15)10RHLCGSRLVEAL2.2
Insulin B(5–15)11RHLCGSHRVEAL>100
Insulin B(5–15)12RHLCGSHLREAL2.0
Insulin B(5–15)13RHLCGSHLVRAL3.0
Insulin B(5–15)14RHLCGSHLVERL52
Insulin B(5–15)15RHLCGSHLVEAR1.4
  • a Binding was measured as described for the DQ0602 competition assay in Materials and Methods.