Table I.

PACAP 38 modulates cytokine release by macrophagesa

Experiment1234
TNF-α (ng/ml)
Medium0000
LPS0.340.161.130.55
PACAP0000
PACAP + LPS0.130.040.700.20
IL-6 (ng/ml)
Medium0000
LPS1.995.361.612.96
PACAP0.551.080.781.55
PACAP + LPS3.056.381.923.49
  • a BALB/c macrophages were preincubated for 2 h in the presence or absence of 10 ng/ml of PACAP 38. LPS (500 ng/ml) was added, and supernatants were collected after 4 h for TNF-α and IL-6 and tested by ELISA. Using the Mann-Whitney test, the decrease in TNF-α and increase in IL-6 when PACAP is preincubated with macrophages before adding LPS is significant, p < 0.05.