Table II.

Binding of modified Melan-A peptides to HLA-A*0201 allelea

PeptideSequenceCompetitor Activityb [μM] 50%Relative Competitor Activityc
Melan-A27–35AAGIGILTV601
ALGIGILTV1.540
AMGIGILTV230
LAGIGILTV651
MAGIGILTV551
Melan-A26–35EAAGIGILTV154
ELAGIGILTV6.59
EMAGIGILTV203
EALGIGILTV1000.6
EAMGIGILT1000.6
YAAGIGILTV415
FAAGIGILTV230
Influenza A matrix58–66GILGFVFTL160
MAGE-3168–176EVDPIGHLY>100<0.6
  • Data reported are from one of three independent experiments giving comparable results.

  • b Competitor activity was measured on the basis of the inhibition of recognition of the MAGE-3271–279 antigenic peptide in the context of HLA-A*0201 by the peptide-specific CTL line 198NS.

  • c The relative competitor activity was calculated as described in Materials and Methods using the Melan-A27–35 as the reference peptide with an arbitrary competitor activity of 1.