Table I.

Identification of the peptide(s) recognized by HLA-A2-restricted, Melan-A specific TILN

Peptide SequenceTILN LAU 203TILN LAU 132
Peptidea [nM] 50%Relative ActivitybPeptide [nM] 50%Relative Activity
AAGIGILTV27–35401151
EAAGIGILTV26–351.527115
AAGIGILTVI27–366000.063000.05
ILTVILGVL32–40>104<4 × 10–3>104<1.5 × 10–3
  • a T2 cells were incubated with various concentrations of the Melan-A peptides 27–35, 26–35, 27–36, and 32–40. Lysis by TILN LAU 203 and LAU 132 was measured by a 4-h 51Cr release cytotoxicity assay at a lymphocyte to target cell ratio of 30:1. The peptide concentration giving 50% of maximal activity, [nM] 50%, was then determined from the titration curve for each peptide.

  • b The relative antigenic activity for each peptide compared with that of the nonapeptide Melan-A27–35 was calculated as described in Materials and Methods.