Table I.

Quantification of catalytically active membrane-bound cathepsin G expressed by activated PMN

DonoraCathepsin G Activity
SAAF-AFCbHPLCc
1154.0 ± 6.9d145.5 ± 4.9
2186.2 ± 7.0196.1 ± 0.8
3320.2 ± 4.2285.3 ± 3.6
4210.7 ± 6.7229.0 ± 1.7
Mean (SD)217.8 ± 72.1214.0 ± 58.7
  • a PMN from four donors were primed for 1 min at 37°C with PAF (10−9 M), then stimulated for 30 min with FMLP (10−8 M). Cells were fixed with 3% paraformaldehyde and 0.5% glutaraldehyde.

  • b Cathepsin G activity expressed by fixed activated cells was assayed in triplicate using SAAF-AFC, as described in Materials and Methods.

  • c Cathepsin G activity expressed by fixed activated cells was assayed in triplicate by HPLC using ANGI as the substrate, as described in Materials and Methods.

  • d Data are ng of cathepsin G per 106 cells (mean ± SD).