Table I.

NO synthesis and toxoplasmastatic activity of WT and TNFR KO macrophagesa

WTKO
Medium1 U/ml IFN-γ100 U/ml IFN-γMedium1 U/ml IFN-γ100 U/ml IFN-γ
Medium6.3 ± 1.6b5.6 ± 1.521.7 ± 5.26.3 ± 0.65.5 ± 1.18.7 ± 1.8
Medium + RH6.3 ± 1.077.7 ± 13.695.2 ± 8.15.5 ± 0.422.2 ± 16.081.7 ± 5.3
% Inhibition0c939402990
Normal IgG5.0 ± 0.94.9 ± 1.116.1 ± 2.36.1 ± 1.05.1 ± 0.66.2 ± 1.5
Normal IgG+ RH5.3 ± 1.879.4 ± 16.286.6 ± 15.55.8 ± 0.523.4 ± 19.582.6 ± 3.7
% Inhibition0849001788
Anti-TNF-α5.2 ± 0.76.2 ± 1.96.5 ± 0.75.7 ± 1.15.6 ± 0.26.9 ± 0.6
Anti-TNF-α + RH5.8 ± 0.912.8 ± 2.268.5 ± 2.76.0 ± 0.312.3 ± 2.478.1 ± 5.2
% Inhibition0227801679
  • a The values shown for % Inhibition and NO synthesis were averaged from each of three mice assayed separately.

  • b Nitric oxide production (nitrite in micromoles per liter).

  • c Inhibition of [3H]uracil uptake: [1− {[(IFN + RH) − (IFN-RH)/(medium + RH)−(medium-RH)}] × 100%.