Table I.

Tolerance among HA-specific CTLp from transgenic Ins-HA mice

EffectorsE:T% Specific Lysis of B10.D2 (H-2d) Target Cells
Alone+PR8+KdNP+KdHA+Vacc-WT+Vacc-HA
BALB/c1074768501149
32404022129
1119129011
Ins-HA1013406361213
3420323610
12511607
  • Ins-HA mice and conventional BALB/c mice were immunized i.p. with 1200 HA units of influenza virus A/PR/8 21 days prior to sacrifice. Spleen cells were cultured with PR8-infected, irradiated homologous APCs. On day 6 cells were assayed for the presence of HA-specific CTL by lysis of virus-infected and peptide-pulsed B10.D2 (H-2d) target cells as indicated. Data are representative of two experiments, each utilizing two mice per group.