Table I.

Peripheral blood analysis of germfree IL2−/− micea

Age (weeks)IL-2 GenotypenRBC (106/μl)HB (mg/dl)HTWBC (103/μl)Differential (×109/L)
NeutLymphMonoEosin
3–5+/+57.9 ± 0.915.0 ± 0.533 ± 17.6 ± 1.50.6 ± 0.26.2 ± 1.10.1 ± 0.10.08 ± 0.02
+/−37.2 ± 1.214.8 ± 0.332 ± 26.8 ± 2.00.7 ± 0.26.4 ± 1.30.2 ± 0.10.09 ± 0.03
−/−47.4 ± 0.813.1 ± 1.128 ± 58.0 ± 2.60.3 ± 0.2b7.0 ± 1.40.1 ± 0.10.1 ± 0.04
8+/+38.6 ± 0.214.2 ± 0.731 ± 111.0 ± 1.91.7 ± 0.39.7 ± 2.50.2 ± 0.20.1 ± 0.06
+/−68.9 ± 0.915.6 ± 1.031 ± 210.1 ± 2.11.6 ± 0.38.6 ± 2.60.2 ± 0.10.1 ± 0.02
−/−43.8 ± 0.610.0 ± 0.519 ± 69.8 ± 2.50.6 ± 0.6c6.3 ± 2.2b0.6 ± 0.20.2 ± 0.05
12+/−49.9 ± 1.914.9 ± 1.833 ± 413.1 ± 2.11.8 ± 0.311.0 ± 3.00.2 ± 0.10.2 ± 0.1
−/−72.6 ± 2.53.7 ± 2.312 ± 314.0 ± 2.20.2 ± 0.2d5.9 ± 2.0b0.6 ± 0.20.3 ± 0.2
  • a HB, hemaglobin; HT, hematocrit; WBC, white blood cells. Data represent the mean ± SD. Manual 300-count leukocyte differentials were performed on blood smears from sex-matched littermates. Data represent the mean ± SD.

  • b p < 0.01 compared with +/+ mice.

  • c p < 0.005 compared with +/+ mice.

  • d p < 0.0005 compared with +/+ mice.

  • b Cells consisted of atypical blast-like cells that were tartrate sensitive, acid phosphatase-positive, and myeloperoxidase-negative.