Table III.

Effect of anti-IL-4 mAb on pulmonary metastasis in BF10 mice or BF1 mice inoculated with BF10-Th2 cells

MiceNo. of Pulmonary MetastasesaReduction (%)
BF10 mice receivedb
Saline (control)244 ± 220
Rat Ig226 ± 377
Anti-IL-4 mAb37 ± 16c85
BF1 mice receivedd
Saline19 ± 5
BF10-Th2 cells (control)347 ± 580
BF10-Th2 cells and rat Ig326 ± 696
BF10-Th2 cells and anti-IL-4 mAb60 ± 33c83
  • a An average number of metastatic colonies per lungs of mice (10 mice each). Results are mean ± SE.

  • b At 4 and 6 days after inoculation with B16F10 melanoma cells (2 × 105 cells/mouse), mice (BF10 mice) were treated i.p. with a 250-μg/kg dose of anti-IL-4 mAb. As controls, the same mice were treated with saline or rat Ig (250 μg/kg).

  • c p < 0.01 vs an appropriate control.

  • d Three days after the implantation of B16F1 melanoma cells (2 × 105 cells/mouse), mice (BF1 mice) were treated with saline or inoculated with 5 × 106 cells/mouse of BF10-Th2 cells. BF10-Th2 cells were CD4+ T cells prepared from spleens of mice at 14 days after the inoculation of 2 × 105 cells/mouse of B16F10 melanoma cells. BF1 mice inoculated with BF10-Th2 cells were treated i.p. with a 250-μg/kg dose of anti-IL-4 mAb or rat Ig 4 and 6 days after the tumor implantation.