Table II.

Phenotypic analysis of BF10-Th2 cells

CellsaIL-4 (pg/ml)bDecrease (%)
Naive CD4+ T cells<50
BF10-Th2 cells (Th2 cells)2515 ± 4220
Th2 cells depleted of CD11b+ cells120 ± 50c95
Th2 cells depleted of CD28+ cells87 ± 31c97
Th2 cells depleted of TCR-αβ+ cells58 ± 15c98
Th2 cells depleted of TCR-γδ+ cells2380 ± 3605
  • a BF10-Th2 cells, CD4+ T cells prepared from mice at 14 days after inoculation of B16F10 melanoma cells (2 × 105 cells/mouse), were treated with various mAbs followed by complement, as described in text.

  • b All cell preparations were stimulated with anti-CD3 mAb (2.5 μg/ml) for 48 h to induce IL-4 production. Culture fluids were assayed for IL-4 by ELISA. The results expressed are the mean ± SD of triplicate determinations.

  • c Student’s t test, p < 0.001 vs original Th2 cells.