Table I.

Cytokine profiles of BF10-Th2 cells

Cell PreparationCytokinesa
IL-2 (U/ml)IFN-γ (pg/ml)IL-4 (pg/ml)IL-10 (pg/ml)
Naive CD4+ T cellsb220 ± 37560 ± 75<50<30
CD4+ T cells from BF1-micec229 ± 34495 ± 91<50<30
BF10-Th2 cellsd<10<302670 ± 1501500 ± 420
  • a Cell preparations were stimulated with anti-CD3 mAb (2.5 μg/ml) for 48 h to induce cytokine production. The culture fluids were assayed for cytokines by ELISA. The results expressed are the mean ± SD of triplicate determinations.

  • b Naive CD4+ T cells were obtained from spleens of normal mice.

  • c CD4+ T cells were prepared from mice 14 days after inoculation of 2 × 105 cells/mouse (i.v.) of B16F1 melanoma cells.

  • d BF10-Th2 cells were CD4+ T cells from spleens of mice at 14 days after inoculation of B16F10 melanoma cells (2 × 105 cells/mouse).