Table I.

IFN-γ production in TNFR-deficient micea

Mouse GenotypeIFN-γ (ng/ml)
2 wk14 wk
wt (B6(7))
Medium<0.0070.24 ± 0.61
SLA10.4 ± 7.821.8 ± 10.2
TNFRp55−/−
Medium<0.0070.9 ± 0.13
SLA8.7 ± 7.3929.1 ± 9.5
wt (129/B6)
Medium<0.0070.05 ± 0.06
SLA1.9 ± 2.02.5 ± 1.0
TNFRp75−/−
Medium<0.0070.09 ± 0.13
SLA2.1 ± 1.85.1 ± 3.4
TNFRp55p75−/−
Medium0.17 ± 0.150.52 ± 0.61
SLA1.3 ± 1.09.5 ± 3.8
  • a Popliteal lymph node cells were cultured with medium or were stimulated with SLA for 72 h after harvesting cells. Quantiation of IFN-γ and IL-4 in the supernatants from 72-h cultures was performed by ELISA. IL-4 levels were equivalent to wt controls and were below 8 U/ml (data not shown). Data are representative of three similar experiments. Values represent the mean and SD for four to five mice. No significant differences (p > 0.05) were seen in IFN-γ production between TNFRp55−/− mice and wt (B6(7)) controls, nor were there significant differences between the wt (129/B6) and TNFRp75−/− or the TNFRp55p75−/− mice.