Table I.

Effect of Fyn on the proportions of thymocyte subsets in TCR transgenic micea

MutationNo. of Thymocytes × 10−7 (mean ± SEM)% of Total Thymocytes (mean ± SEM)
CD48CD4+8+CD4+8CD48+
H-Y7.8 ± 1.018 ± 2.358 ± 3.48.5 ± 0.616 ± 1.4
H-Y/Fyn−/−12.7 ± 2.110 ± 1.767 ± 2.09.1 ± 0.614 ± 1.7
2C1.7 ± 0.245 ± 3.622 ± 2.914.8 ± 2.218 ± 2.9
2C/Fyn−/−4.2 ± 0.621 ± 3.946 ± 3.26.0 ± 1.227 ± 2.3
  • a Six mice (6–12 wk old) were analyzed per group. Thymocytes from H-2b 2C TCR transgenic mice with or without the Fyn−/− mutation or female H-2b H-Y TCR transgenic mice with or without the Fyn−/− mutation were stained with PE-labeled anti-CD4 and FITC-labeled anti-CD8 mAbs and analyzed in the FACScan flow cytometer. The total number of viable thymocytes recovered from these transgenic lines is indicated.