Table III.

In vivo effect of 1,25-(OH)2D3 on cytokine transcript number

Micea
Control1,25-(OH)2D3
Serum Ca8.4 ± 0.49.2 ± 0.4
TGF-β1/1000 G3PDH38 ± 11190 ± 51b
IL-4/1000 G3PDH729 ± 2802815 ± 1105
IFN-γ/1000 G3PDH7.4 ± 1.57.3 ± 1.9
TNF-α/1000 G3PDH17 ± 1034 ± 11
  • a Control immunized mice with EAE severity scores of 2.5 were randomly split into two groups and 1,25-(OH)2D3 or control injected. Twenty-four hours later animals were sacrificed and the LN and serum of both groups of animals were saved for analyses. Values are the mean ± SEM of five mice.

  • b 1,25-(OH)2D3 treatment significantly increased the production of TGF-β1 compared to the controls.