Table II.

1,25-(OH)2D3 in vivo increases IL-4 and TGF-β1 mRNA in spinal cord and brain samplesa

Control1,25-(OH)2D3
IFN-γ/1000 G3PDH85 ± 1542 ± 11
TNF-α/1000 G3PDH9 ± 320 ± 5
TGF-β1/1000 G3PDH27 ± 8171 ± 49b
IL-4/1000 G3PDH0 ± 057 ± 0.3b
  • a QC-PCR of mRNA from the spinal cord and brain of control and 1,25-(OH)2D3-treated mice. Samples were taken when the EAE severity of control mice was ≥2.5. Values are the mean ± SEM of triplicate experiments. Each experiment used spinal cords and brains from six to eight pooled mice.

  • b Values from 1,25-(OH)2D3-treated mice are significantly higher than the controls; p = 0.0002.