Table II.

Failure of CD3 + CD28 coligation to induce apoptosis in Jurkat tTA cellsa

Hours of StimulationPercent Apoptosis Following Stimulation with
Anti-CD3Anti-CD3 + anti-CD28
04.8 ± 1.84.3 ± 1.5
1215.5 ± 2.33.4 ± 5.2
2428.3 ± 4.63.4 ± 3.4
4841.7 ± 2.56.1 ± 1.8
7243.1 ± 1.87.9 ± 4.0
  • a Jurkat tTA cells were stimulated with 10 μg/ml anti-CD3 (OKT3) or a combination of 10 μg/ml anti-CD3 + 2 μg/ml anti-CD28 (9.3) for 24 h. Cell death was determined by trypan blue exclusion. Duplicate counts were performed by two independent observers.