Table III.

Lymphocyte expression of L-selectin or α4β7 integrina

Tissue:% of Cells That Are L-selectin+ (MFI of expression)b% of Cells That Are α4β7+ (MFI of expression)
B cellsT cellsCD4+ cellsCD8+ cellsB cellsT cellsCD4+cellsCD8+cells
Blood96 ± 196 ± 196 ± 199 ± 196 ± 178 ± 4c90 ± 291 ± 1
(102 ± 8)(163 ± 15)(138 ± 13)(178 ± 19)(42 ± 3)(30 ± 2)(29 ± 5)(33 ± 3)
PLN88 ± 694 ± 388 ± 496 ± 3c97 ± 176 ± 273 ± 179 ± 1
(102 ± 14)(146 ± 15)(113 ± 9)(151 ± 16)(34 ± 1)(17 ± 1)(16 ± 1)(18 ± 2)
MLN83 ± 689 ± 282 ± 397 ± 197 ± 176 ± 475 ± 381 ± 2
(87 ± 15)(146 ± 12)(117 ± 10)(161 ± 17)(34 ± 1)(19 ± 1)(19 ± 1)(19 ± 1)
Peyer’s patch82 ± 346 ± 336 ± 271 ± 498 ± 296 ± 472 ± 485 ± 2
(76 ± 10)(125 ± 11)(105 ± 11)(141 ± 15)c(32 ± 1)(21 ± 1)(21 ± 1)(21 ± 1)
Spleen78 ± 378 ± 472 ± 294 ± 285 ± 273 ± 272 ± 282 ± 3c
(88 ± 9)(164 ± 13)(129 ± 9)(180 ± 9)(28 ± 1)(20 ± 1)(20 ± 1)(20 ± 2)
  • a Adhesion molecule expression by lymphocyte subsets of wild-type mice was assessed by two-color immunofluorescence cytometry as in Figure 3. All values represent the means ± SEM obtained for lymphocytes from three or more mice.

  • b MFI, mean fluorescence intensity. Values were obtained by determining the mean linear fluorescence channel numbers for cells expressing each adhesion molecule.

  • c Differences between B and T cells or CD4+ and CD8+ T cells were significant, p<0.05; p<0.01.