RT Journal Article
SR Electronic
T1 Role of CD25<sup>+</sup> Dendritic Cells in the Generation of Th17 Autoreactive T Cells in Autoimmune Experimental Uveitis
JF The Journal of Immunology
JO J. Immunol.
FD American Association of Immunologists
SP 5785
OP 5791
DO 10.4049/jimmunol.1200109
VO 188
IS 11
A1 Liang, Dongchun
A1 Zuo, Aijun
A1 Shao, Hui
A1 Born, Willi K.
A1 O’Brien, Rebecca L.
A1 Kaplan, Henry J.
A1 Sun, Deming
YR 2012
UL http://www.jimmunol.org/content/188/11/5785.abstract
AB In the current study, we showed that in vivo administration of an anti-CD25 Ab (PC61) decreased the Th17 response in C57BL/6 mice immunized with the uveitogenic peptide interphotoreceptor retinoid-binding protein (1–20), while enhancing the autoreactive Th1 response. The depressed Th17 response was closely associated with decreased numbers of a splenic dendritic cell (DC) subset expressing CD11c+CD3−CD25+ and decreased expansion of γδ T cells. We demonstrated that ablation of the CD25+ DC subset accounted for the decreased activation and the expansion of γδ T cells, leading to decreased activation of IL-17+ interphotoreceptor retinoid-binding protein-specific T cells. Our results show that an enhanced Th17 response in an autoimmune disease is associated with the appearance of a DC subset expressing CD25 and that treatment of mice with anti-CD25 Ab causes functional alterations in a number of immune cell types, namely DCs and γδ T cells, in addition to CD25+αβTCR+ regulatory T cells.