RT Journal Article
SR Electronic
T1 Studies on the Role of Ca<sup>++</sup> and Mg<sup>++</sup> in Complement Fixation and Immune Hemolysis
JF The Journal of Immunology
JO J. Immunol.
FD American Association of Immunologists
SP 374
OP 379
VO 71
IS 5
A1 Levine, Lawrence
A1 Osler, Abraham G.
A1 Mayer, Manfred M.
YR 1953
UL http://www.jimmunol.org/content/71/5/374.abstract
AB 1. It has been shown that Ca++ as well as Mg++ are essential for immune hemolysis. 2. Mg++ can be replaced with a fair degree of efficiency by Co++ or Ni++. The requirement for Ca++ could not be met by Co++, Mn++, Ni++, Mg++, Cd++, Sr++, Ba++, and Zn++. 3. Evidence is presented showing that immune hemolysis takes place in at least two reaction steps: Step 1, involving C′ fixation requires Ca++; Step 2 is a reaction of an unknown nature, which requires Mg++.