RT Journal Article
SR Electronic
T1 CD8<sup>+</sup> Cell Depletion Accelerates HIV-1 Immunopathology in Humanized Mice
JF The Journal of Immunology
JO J. Immunol.
FD American Association of Immunologists
SP 7082
OP 7091
DO 10.4049/jimmunol.1000438
VO 184
IS 12
A1 Gorantla, Santhi
A1 Makarov, Edward
A1 Finke-Dwyer, Jennifer
A1 Gebhart, Catherine L.
A1 Domm, William
A1 Dewhurst, Stephen
A1 Gendelman, Howard E.
A1 Poluektova, Larisa Y.
YR 2010
UL http://www.jimmunol.org/content/184/12/7082.abstract
AB Stable engraftment of human lymphoid tissue in NOD/scid–IL-2Rγcnull mice after CD34+ hematopoietic stem cell reconstitution permits the evaluation of ongoing HIV-1 infection for weeks to months. We demonstrate that HIV-1–infected rodents develop virus-specific cellular immune responses. CD8+ cell depletion, 2 or 5–7 wk after viral infection, resulted in a significant increase of HIV-1 load, robust immune cell activation, and cytopathology in lymphoid tissues but preserved CD4/CD8 double-positive thymic T cell pools. Human CD8+ cells reappeared in circulation as early as 2–3 wk. These data support a role of CD8+ cells in viral surveillance and the relevance of this humanized mouse model for the studies of HIV-1 pathobiology and virus-specific immunity.