PT  - JOURNAL ARTICLE
AU  - Makita, Shin
AU  - Kanai, Takanori
AU  - Nemoto, Yasuhiro
AU  - Totsuka, Teruji
AU  - Okamoto, Ryuichi
AU  - Tsuchiya, Kiichiro
AU  - Yamamoto, Masafumi
AU  - Kiyono, Hiroshi
AU  - Watanabe, Mamoru
TI  - Intestinal Lamina Propria Retaining CD4<sup>+</sup>CD25<sup>+</sup> Regulatory T Cells Is A Suppressive Site of Intestinal Inflammation
AID  - 10.4049/jimmunol.178.8.4937
DP  - 2007 Apr 15
TA  - The Journal of Immunology
PG  - 4937--4946
VI  - 178
IP  - 8
4099  - http://www.jimmunol.org/content/178/8/4937.short
4100  - http://www.jimmunol.org/content/178/8/4937.full
SO  - J. Immunol.2007 Apr 15; 178
AB  - It is well known that immune responses in the intestine remain in a state of controlled inflammation, suggesting that not only does active suppression by regulatory T (TREG) cells play an important role in the normal intestinal homeostasis, but also that its dysregulation of immune response leads to the development of inflammatory bowel disease. In this study, we demonstrate that murine CD4+CD25+ T cells residing in the intestinal lamina propria (LP) constitutively express CTLA-4, glucocorticoid-induced TNFR, and Foxp3 and suppress proliferation of responder CD4+ T cells in vitro. Furthermore, cotransfer of intestinal LP CD4+CD25+ T cells prevents the development of chronic colitis induced by adoptive transfer of CD4+CD45RBhigh T cells into SCID mice. When lymphotoxin (LT)α-deficient intercrossed Rag2 double knockout mice (LTα−/− × Rag2−/−), which lack mesenteric lymph nodes and Peyer’s patches, are transferred with CD4+CD45RBhigh T cells, they develop severe wasting disease and chronic colitis despite the delayed kinetics as compared with the control LTα+/+ × Rag2−/− mice transferred with CD4+CD45RBhigh T cells. Of note, when a mixture of splenic CD4+CD25+ TREG cells and CD4+CD45RBhigh T cells are transferred into LTα−/− × Rag2−/− recipients, CD4+CD25+ TREG cells migrate into the colon and prevent the development of colitis in LTα−/− × Rag2−/− recipients as well as in the control LTα+/+ × Rag2−/− recipients. These results suggest that the intestinal LP harboring CD4+CD25+ TREG cells contributes to the intestinal immune suppression.