PT  - JOURNAL ARTICLE
AU  - Purbhoo, Marco A.
AU  - Sutton, Deborah H.
AU  - Brewer, Joanna E.
AU  - Mullings, Rebecca E.
AU  - Hill, Maxine E.
AU  - Mahon, Tara M.
AU  - Karbach, Julia
AU  - Jäger, Elke
AU  - Cameron, Brian J.
AU  - Lissin, Nikolai
AU  - Vyas, Paresh
AU  - Chen, Ji-Li
AU  - Cerundolo, Vincenzo
AU  - Jakobsen, Bent K.
TI  - Quantifying and Imaging NY-ESO-1/LAGE-1-Derived Epitopes on Tumor Cells Using High Affinity T Cell Receptors
AID  - 10.4049/jimmunol.176.12.7308
DP  - 2006 Jun 15
TA  - The Journal of Immunology
PG  - 7308--7316
VI  - 176
IP  - 12
4099  - http://www.jimmunol.org/content/176/12/7308.short
4100  - http://www.jimmunol.org/content/176/12/7308.full
SO  - J. Immunol.2006 Jun 15; 176
AB  - Presentation of intracellular tumor-associated Ags (TAAs) in the context of HLA class I molecules offers unique cancer-specific cell surface markers for the identification and targeting of tumor cells. For most peptide Ags, the levels of and variations in cell surface presentation remain unknown, yet these parameters are of crucial importance when considering specific TAAs as targets for anticancer therapy. Here we use a soluble TCR with picomolar affinity for the HLA-A2-restricted 157–165 epitope of the NY-ESO-1 and LAGE-1 TAAs to investigate presentation of this immunodominant epitope on the surface of a variety of cancer cells. By single molecule fluorescence microscopy, we directly visualize HLA-peptide presentation for the first time, demonstrating that NY-ESO-1/LAGE-1-positive tumor cells present 10–50 NY-ESO-1/LAGE-1157–165 epitopes per cell.