RT Journal Article
SR Electronic
T1 Involvement of CCL18 in Allergic Asthma
JF The Journal of Immunology
JO J. Immunol.
FD American Association of Immunologists
SP 6286
OP 6293
DO 10.4049/jimmunol.176.10.6286
VO 176
IS 10
A1 Nadaï, Patricia de
A1 Charbonnier, Anne-Sophie
A1 Chenivesse, Cécile
A1 Sénéchal, Stéphanie
A1 Fournier, Clément
A1 Gilet, Jules
A1 Vorng, Han
A1 Chang, Ying
A1 Gosset, Philippe
A1 Wallaert, Benoı̂t
A1 Tonnel, André-Bernard
A1 Lassalle, Philippe
A1 Tsicopoulos, Anne
YR 2006
UL http://www.jimmunol.org/content/176/10/6286.abstract
AB Allergic asthma is associated with a pulmonary recruitment of Th type 2 cells, basophils, and eosinophils, mainly linked to chemokine production. CCL18 is a chemokine preferentially expressed in the lung, secreted by APCs, induced by Th2-type cytokines, and only present in humans. Therefore, CCL18 may be involved in allergic asthma. PBMC from asthmatics allergic to house dust mite cultured in the presence of Dermatophagoides pteronyssinus 1 (Der p 1) allergen secreted CCL18, 48 and 72 h after stimulation, whereas those from healthy donors did not. Part of CCL18 was directly derived from Der p 1-stimulated plasmacytoid dendritic cells, whereas the other part was linked to monocyte activation by IL-4 and IL-13 produced by Der p 1-stimulated T cells. In bronchoalveolar lavages from untreated asthmatic allergic patients, CCL18 was highly increased compared with controls. Functionally, CCL18 preferentially attracted in vitro-polarized Th2 cells and basophils, but not eosinophils and Th1 cells, and induced basophil histamine and intracellular calcium release. These data show a new function for CCL18, i.e., the recruitment of Th2 cells and basophils, and suggest that CCL18 may play a predominant role in allergic asthma.