RT Journal Article
SR Electronic
T1 Cutting Edge: Generation of Splenic CD8<sup>+</sup> and CD8<sup>−</sup> Dendritic Cell Equivalents in Fms-Like Tyrosine Kinase 3 Ligand Bone Marrow Cultures
JF The Journal of Immunology
JO J. Immunol.
FD American Association of Immunologists
SP 6592
OP 6597
DO 10.4049/jimmunol.174.11.6592
VO 174
IS 11
A1 Naik, Shalin H.
A1 Proietto, Anna I.
A1 Wilson, Nicholas S.
A1 Dakic, Aleksandar
A1 Schnorrer, Petra
A1 Fuchsberger, Martina
A1 Lahoud, Mireille H.
A1 O’Keeffe, Meredith
A1 Shao, Qi-xiang
A1 Chen, Wei-feng
A1 Villadangos, José A.
A1 Shortman, Ken
A1 Wu, Li
YR 2005
UL http://www.jimmunol.org/content/174/11/6592.abstract
AB We demonstrate that functional and phenotypic equivalents of mouse splenic CD8+ and CD8− conventional dendritic cell (cDC) subsets can be generated in vitro when bone marrow is cultured with fms-like tyrosine kinase 3 (flt3) ligand. In addition to CD45RAhigh plasmacytoid DC, two distinct CD24high and CD11bhigh cDC subsets were present, and these subsets showed equivalent properties to splenic CD8+ and CD8− cDC, respectively, in the following: 1) surface expression of CD11b, CD24, and signal regulatory protein-α; 2) developmental dependence on, and mRNA expression of, IFN regulatory factor-8; 3) mRNA expression of TLRs and chemokine receptors; 4) production of IL-12 p40/70, IFN-α, MIP-1α, and RANTES in response to TLR ligands; 5) expression of cystatin C; and 6) cross-presentation of exogenous Ag to CD8 T cells. Furthermore, despite lacking surface CD8 expression, the CD24high subset contained CD8 mRNA and up-regulated surface expression when transferred into mice. This culture system allows access to bona fide counterparts of the splenic DC subsets.