PT  - JOURNAL ARTICLE
AU  - Naik, Shalin H.
AU  - Proietto, Anna I.
AU  - Wilson, Nicholas S.
AU  - Dakic, Aleksandar
AU  - Schnorrer, Petra
AU  - Fuchsberger, Martina
AU  - Lahoud, Mireille H.
AU  - O’Keeffe, Meredith
AU  - Shao, Qi-xiang
AU  - Chen, Wei-feng
AU  - Villadangos, José A.
AU  - Shortman, Ken
AU  - Wu, Li
TI  - Cutting Edge: Generation of Splenic CD8<sup>+</sup> and CD8<sup>−</sup> Dendritic Cell Equivalents in Fms-Like Tyrosine Kinase 3 Ligand Bone Marrow Cultures
AID  - 10.4049/jimmunol.174.11.6592
DP  - 2005 Jun 01
TA  - The Journal of Immunology
PG  - 6592--6597
VI  - 174
IP  - 11
4099  - http://www.jimmunol.org/content/174/11/6592.short
4100  - http://www.jimmunol.org/content/174/11/6592.full
SO  - J. Immunol.2005 Jun 01; 174
AB  - We demonstrate that functional and phenotypic equivalents of mouse splenic CD8+ and CD8− conventional dendritic cell (cDC) subsets can be generated in vitro when bone marrow is cultured with fms-like tyrosine kinase 3 (flt3) ligand. In addition to CD45RAhigh plasmacytoid DC, two distinct CD24high and CD11bhigh cDC subsets were present, and these subsets showed equivalent properties to splenic CD8+ and CD8− cDC, respectively, in the following: 1) surface expression of CD11b, CD24, and signal regulatory protein-α; 2) developmental dependence on, and mRNA expression of, IFN regulatory factor-8; 3) mRNA expression of TLRs and chemokine receptors; 4) production of IL-12 p40/70, IFN-α, MIP-1α, and RANTES in response to TLR ligands; 5) expression of cystatin C; and 6) cross-presentation of exogenous Ag to CD8 T cells. Furthermore, despite lacking surface CD8 expression, the CD24high subset contained CD8 mRNA and up-regulated surface expression when transferred into mice. This culture system allows access to bona fide counterparts of the splenic DC subsets.